王聖帆 副教授

12

Sheng-Fan Wang, Associate Professor

  • 研究室:濟世大樓 CS607 / 國際學術大樓-傳染病與癌症研究中心
  • 單位電話:(07)-312-1101 ext. 2558 or 2354
  • 電子信箱:wasf1234@kmu.edu.tw

學歷

  • 國立陽明大學 醫學生物技術暨檢驗學系 博士 (2005-2009)
  • 國立陽明大學 醫學生物技術研究所 碩士 (1999-2001)
  • 國立陽明大學 醫事技術學系 學士 (1995-1999)

執照與證書

  • 醫事檢驗師執照
  • 第二級與第三級生物實驗室使用證書
  • ISO 15189 實驗室人員認證
  • 人體試驗倫理課程通過證書

經歷

  • 中央研究院 生物醫學研究所 博士後研究 (2010-2014)
  • 國立陽明大學 醫學生物技術暨檢驗學系 博士後研究 (2009-2010)
  • 行政院衛生部疾病管制署 國防訓儲人員 (2001-2005)

教學科目

  • 免疫學
  • 臨床病毒學
  • 臨床微生物學
  • PBL問題導向學習課程
  • 微生物學與微生物實驗
  • 臨床實習教學
  • Seminar (Postgraduates)
  • Special Topics of Medical Virology (Postgraduates)
  • Special Topics of Virological and Immunological Researches in HIV-1 and Influenza Viruses (Postgraduates)

獎勵

  • The invited Lead Guest Editor of Canadian Journal of Infectious Diseases and Medical Microbiology Journal, 2017
  • The scholarship award from International AIDS Conference, 2016
  • Best Tutor Award in the Department of Laboratory Science and Biotechnology, 2016
  • The best poster award of annual conference of Taiwan Society of Microbiology, 2014
  • The best research award, IBMS, Academia Sinica, Taiwan, 2013.
  • The Phi-Tau-Phi Scholastic Honor, 2009
  • The scholarship award from International AIDS Conference, 2004

受邀演講與授課

 

2014

Kaohsiung Medical University/ Center for Infectious Disease and Cancer Research.

(The 11th Guangdong, Hong Kong, Macau & Taiwan Conference on Preventive Medicine)

Taiwan

2015

Kaohsiung Medical University/School of Dentistry

Taiwan

2017

HIV-1 Club for clinical physician

Taiwan

 

研究興趣

本實驗室主要研究病毒相關的傳染病與追溯病毒來源及可能的機制,目前著重於新興及在浮現之傳染病。研究的主題包括: 半乳醣凝集素(Galectin)在病毒感染的影響及機制、登革熱病毒造成流行的原因以及登革血熱的相關機制探討、茲卡(Zika)病毒與登革熱病毒之間的交互作用與影響、流感病毒包括:人流感 H1N1,H3N2與禽流感病毒H5N1, H7N9之血球凝集素(Hemagglutinin)單株抗體的備製與分析抗體預防性與治療性之特性。實驗室目前的合作單位包括:中央研究院生物醫學研究所、美國NIH、德國科隆大學(Cologne University)、泰國瑪希敦大學(Marhidol University)與朱拉隆功大學(Chulalongkorn University)。

執行之研究計畫

研究題目

執行期間

補助金額

補助單位

1. Muti-resolutions 3D visualization of HIV-1 NL4-3 and CRF07_BC viral attachment and internalization kinetics to CD4+ T cells as well as application for anti-HIV drug screening platform development(1/3).

2013-08-01

to

2014-07-31

800,000 NT

Center for Infectious Disease and Cancer Research, Kaohsiung Medical University, Grants No.

KMUTP103E03

2. Establishment of HIV-1 drug resistance and functional cure evaluation platform

2013-08-01

to

2014-07-31

300,000 NT

Center for Infectious Disease and Cancer Research, Kaohsiung Medical University, Grants No. KMU- TP103E20

3. The study of the role of truncated HIV CRF07_BC p6Gag in virus maturation, budding and disease progression

2015-01-01

to

2015-12-31

500,000 NT

Kaohsiung Medical University Research Foundation (KMUQ104001).

4. Muti-resolutions 3D visualization of HIV-1 NL4-3 and CRF07_BC viral attachment and internalization kinetics to CD4+ T cells as well as application for anti-HIV drug screening platform development(2/3).

2015-10-01

to

2016-09-31

1,000,000 NT

Center for Infectious Disease and Cancer Research, Kaohsiung Medical University, Grants No. (KMU-TP104E04)

5.The role of endogenous galectin-3 in virus budding  and  cell-to-cell  transmission of  HIV-1 CRF07_BC viruses(1/3)

2015-08-01

to

2016-07-31

1,700,000 NT

Ministry of Science and Technology, Republic of China (MOST 104-2321-B-037-002).

6. Generating and characterizing mono-and polyclonal antibodies against baculovirus-insect based producing trimeric hemagglutinin of H7N9 avian influenza virus and application for important epitopes mapping on hemagglutinin.

2016-01-01

to

2016-12-31

400,000 NT

Kaohsiung Medical University Research Foundation

(KMU-Q105010)

7. The role of endogenous galectin-3 in virus budding  and  cell-to-cell  transmission of  HIV-1 CRF07_BC viruses(3/3)

2016-08-01

to

2017-07-31

1,700,000 NT

Ministry of Science and Technology, Republic of China (MOST 105-2321-B-037 -001)

8. Muti-resolutions 3D visualization of HIV-1 NL4-3 and CRF07_BC viral attachment and internalization kinetics to CD4+ T cells as well as application for anti-HIV drug screening platform development (2/3).

2016-11-01

to

2017-10-31

600,000 NT

Center for Infectious Disease and Cancer Research, Kaohsiung Medical University, Grants No. (KMU-TP105E04)


專利申請

  • N-terminal fragment of galectin-3 inhibits HIV-1 budding. ( Fu-Tong Liu, Huan-Yuan Chen and Sheng-Fan Wang) (Patent No. 12A-1020422).

國際期刊論文著作 (SCI)

  1. Szu-Hao Kung, Sheng-Fan Wang, Chaio-Wei Huang, Chiu-Chu Hsu, Hsin-Fu Liu, Jyh-Yuan Yang. Genetic and antigenic analyses of enterovirus 71 isolates in Taiwan during 1998-2005. Clin Microbiol Infect. 2007;13(8):782-787.
  2. Sheng-Fan Wang, Jason C. Huang, Yuan-Ming Lee, Shih-Jen Liu, Yu-Jiun Chan, Yat-Pang Chau, Pele Chong and Yi- Ming Arthur Chen. DC-SIGN mediates avian H5N1 influenza virus infection in cis and in trans. Biochem Biophys Res Commun. 2008,373(4):561-566.
  3. Sheng-Fan Wang, Kuan-Hsuan Chen, Arunee Thitithanyanont, Ling Yao, Yuan-Ming, Lee, Yu-Jiun Chan, Shih-Jen Liu, Pele Chong, Wu-Tse Liu, Jason C. Huang, and Yi-Ming Arthur Chen. Generating and Characterizing Monoclonal and Polyclonal Antibodies against Avian H5N1 Hemagglutinin Protein. Biochem Biophys Res Commun. 2009,382(4):691-696.
  4. Sheng-Fan Wang, Yuan-Ming Lee,Yu-Jiun Chan, Hsin-Fu Liu,Yung-Fong Yen, Wu-Tse Liu, Jason C. Huang, Yi- Ming Arthur Chen Influenza A virus in Taiwan, 1980-2006: Phylogenetic and Antigenic Characteristics of the Hemagglutinin Gene. Journal of Medical Virology. 2009 Aug;81(8):1457-70.
  5. Yuan-Ming Lee, Sheng-Fan Wang, Cheng-Ming Lee, Kuan-Hsuan Chen, Yu-Jiun Chan, Wu-Tse Liu and Yi- Ming Athur Chen. Virological investigation of four outbreaks of influenza B reassortants in the northern region of Taiwan from October 2006 to February 2007. BMC research Note. 2009, 9;2(1):86.
  6. Sheng-Fan Wang, Ling Yao, Shih-Jen Liu, Pele Chong, Yi-Ming Arthur Chen and Jason C. Huang. Identifying Conserved DR1501-restricted CD4+ T cell Epitopes in Hemagglutinin Proteins after H5N1 Vaccine Inoculation. (Viral Immunology ; Accepted).
  7. Ying-Feng Chang*, and Sheng-Fan Wang*, Jason C. Huang*,Li-Chen Su, Ying-Chen Li, Cheng-Chung Lee, Ling Yao, Yi-Ming A. Chen, and Chien Chou. Detection of Swine-origin Influenza A (H1N1) Viruses Using a Localized Surface Plasmon Coupled Fluorescence Fiber-optic Biosensor. (*equal contribution) Biosensors and Bioelectronics; 2010 ;26(3):1068-73.
  8. Che-Feng Liao*, Sheng-Fan Wang*,Yu-Ting Lin,and Yi-Ming Arthur Chen. Identification of the DC-SIGN-interactive domains on the envelope glycoprotein of HIV-1 CRF07_BC. (*equal contribution) AIDS Research and Human Retroviruses; 2011, 27(8): 831-839.
  9. Yuan-Ming Lee; Huang-Jie Lin; Yen-Ju Chen; Cheng-Ming Lee; Sheng-Fan Wang; Kai-Yuan Chang; Tzu-Lang Chen; Hsin-Fu Liu; Yi-Ming Arthur Chen. Molecular epidemiology of HCV genotypes among injection drug users in Taiwan: Full-length sequences of two new subtype 6w strains and a recombinant form_2b6w. Journal of medical virology 2010;82(1):57-68
  10. Chun-Wei Lee, Shr-Jeng Jim Leu, Ruei-Ying Tzeng, Sheng-Fan Wang, Shu-Chun Tsai, Kuang-Hui Sun, Ruey-Hwa Chen, Jason C. Huang Latent membrane protein 1 of Epstein–Barr virus regulates death-associated protein kinase 1 in lymphoblastoid cell line. Virology 2011 Apr. 413 (2011) 19–25.
  11. Sheng-Fan Wang, Kuan-Hsuan Chen, Yen-Ju Chen, Wen-Yi Lee, Marcelo Chen, Jason C. Huang, Yi-Ming Arthur Chen. Human-Leukocyte-Antigen Class I Cw 1502 and Class II DR 0301 Genotypes are Associated with Resistance to Severe Acute Respiratory Syndrome (SARS) Infection. Viral Immunology, 2011, 24(5): 421-426.
  12. Hoa Minh Luong*, Sheng-Fan Wang*, Vu Tan Trao, Suh-Chin Wu, Marcelo Chen, Jason C. Huang, Phung Dac Cam, Yi-Ming Arthur Chen. Retrospective survey of avian influenza H5N1 infection in Northern Vietnam by using a combinational serologic assay. (*equal contribution) Health, 4, 974-982.
  13. Lee YM, Chuang SY, Wang SF, Lin YT, Chen YM., Epidemiology of human herpesvirus type 8 and parvovirus B19 infections and their association with HIV-1 among men who have sex with men and injection drug users in Taiwan. J Microbiol Immunol Infect. 2013 Mar 3. pii: S1684-1182(13)00021-2.
  14. Sheng-Fan Wang, Sung-Pin Tseng, Chia-Hung Yen, Jyh-Yuan Yang, Ching-Han Tsao,

    Chun-Wei Shen, Kuan-Hsuan Chen, Fu-Tong Liu, Wu-Tse Liu, Yi-Ming Arthur Chen, Jason C. Huang. Antibody-dependent SARS coronavirus infection is mediated by antibodies against spike proteins. Biochem Biophys Res Commun. 2014 Aug 22;451(2):208-14.

  15. Sheng-Fan Wang, Ching-Han Tsao, Yu-Ting Lin, Daniel K. Hsu, Meng-Lin Chiang,Chia-Hui Lo, Fan-Ching Chien, Peilin Chen, Yi-Ming Arthur Chen, Huan-Yuan Chen,Fu-Tong Liu. Galectin-3 promotes HIV-1 budding via association with Alix and Gagp6. Glycobioilogy 2014 Nov;24(11):1022-35.
  16. Sheng-Fan Wang, Ming-Wei Su, Sung-Pin Tseng, Ching-Han Tsao, Szu-Wei Huang, Woei-Chyn Chu, Wu-Tse Liu, Yi- Ming Arthur Chen6, and Jason C. Huang (2014, Oct). Analysis of codon usage preference in hemagglutinin genes of the swine-origin influenza A (H1N1) virus. J Microbiol Immunol Infect, 2014 Oct 31. pii: S1684-1182(14)00180-7.
  17. Szu-Wei Huang*, Sheng-Fan Wang*, Yu-Ting Lin, Chia-Hung Yen, Chih-Hao Lee, Wing-Wai Wong, Hung-Chin Tsai, Chia-Jui Yang, Bor-Shen Hu, Yu-Huei Lin, Chin-Tien Wang, Jaang-Jiun Wang, Zixin Hu, Daniel R. Kuritzkes, Yen- Hsu Chen, Yi-Ming Arthur Chen (*These authors contributed equally to this work) (2014, Dec). Patients Infected with CRF07_BC Have Significantly Lower Viral Loads than Patients with HIV-1 Subtype B: Mechanism and Impact on Disease Progression. PLoS One, December 2014 11;9(12):e114441
  18. Sheng-Fan Wang, Sung-Pin Tseng, Szu-Wei Huang, Chia-Hung Yen, Yi-Wei Hong, Marcelo Chen,and Yi-Ming Arthur Chen (2015, Feb). Mitochondria Polarization in the Contact Regions of Virus-Infected Effector Cells During Cell-to-Cell Transmission of HIV-1. AIDS RESEARCH AND HUMAN RETROVIRUSES, 2015 Feb;31(2):175-6.
  19. Szu-Wei Huang, Sheng-Fan Wang, Ángel E. Cowó, Marcelo Chen, Yu-Ting Lin, Chun-Po Hung, Yi-Hsien Chen, Jyh-Yuan Yang, Hung-Jen Tang, Yi-Ming Arthur Chen. (2015, May). Molecular Epidemiology of HIV-1 Infection amongMen who Have Sex with Men in Taiwan in 2012.PLoS One, 2015 Jun 3;10(6):e0128266.
  20. Sung-Pin Tseng, Sheng-Fan Wang, Cheng-Yu Kuo, Jun-Wei Huang, Wei-Chun Hung, Guan-Ming Ke, Po-Liang Lu. Characterization of Fosfomycin Resistant Extended- Spectrum β-Lactamase- Producing Escherichia coli Isolates from Human and Pig in Taiwan. PLoS One. 2015 Aug 17;10(8):e0135864.
  21. Sheng-Fan Wang, Ko Chang, Ruo-Wei Lu, Wen-Hung Wang, Yen-Hsu Chen, Marcelo Chen, Deng-Chyang Wu and Yi-Ming Arthur Chen. Large Dengue virus type 1 outbreak in Taiwan. Emerging Microbes & Infections (2015) 4, e46
  22. Sheng-Fan Wang,*(first author and correspondence) Wen-Hung Wang, Ko Chang, Yen-Hsu Chen, Sung-Pin Tseng, Chia-HungYen, Deng-Chyang Wu, and Yi-Ming Arthur Chen*. Severe Dengue Fever Outbreak in Taiwan. Am. J. Trop. Med. Hyg., 2016 Jan;94(1):193-7.
  23. Sheng-Fan Wang*, Sung-Pin Tseng, El-Wui Loh, Wen-Hung Wang, Ming-Chun Li, Kuan-Hsuan Chen, Wan-Chi Tsai, Yuan-Ming Lee, Huan-Yuan Chen, Fu-Tong Liu, Yi-Ming. Arthur Chen, Jason C. Huang. Generation and characterization of new monoclonal antibodies against swine origin 2009 influenza A (H1N1) virus and evaluation of their prophylactic and therapeutic efficacy in a mouse model. Developmental and Comparative Immunology, 2017 Feb;67:8-17 (Zoology: 6/161;3.7% ).
  24. Sheng-Fan Wang, Ko Chang, El-Wui Loh, Wen-Hung Wang, Sung-Pin Tseng, Po-Liang Lu, Yen-Hsu Chen, Yi-Ming Arthur Chen. Consecutive large dengue outbreaks in Taiwan in 2014–2015. Emerging Microbes and Infections.7;5(12):e123. (IF: 4.02)

國內期刊論文著作

  1. Wang S.F , Yang C.Y , Tseng T.C , Chen H.Y. Molecular Biological Analysis of the First Imported Rabies Case in Taiwan. Taiwan Epidemiology Bulletin 2002 ; October 25 , Vol. 18 , No. 10 : 245-255.
  2. Wang S.F , Lin C.L , Yang C.Y. Establishment of Real-time PCR Analysis System to detect Enterovirus infection. Taiwan Epidemiology Bulletin 2002 ; November 25 , Vol. 18 , No. 11 : 267-277.
  3. Hsu L.C , Hsu F.K , Chang S.F , Chou L , Wang S.F , Yang S.Y. Establishing a Yellow Fever Serological Testing Method. Taiwan Epidemiology Bulletin 2003 ; March 25 , Vol. 19 , No. 3 : 47-59.
  4. Wang S.F , Yang C.Y , Lee H.C , Chen. H.Y. Molecular Biological Analysis of SARS Virus in Taiwan. Taiwan Epidemiology Bulletin 2003 ; June 25 , Vol. 19 , No. 6 : 127-141
  5. Wang S.F , Lee H.C , Chen H.Y , Yang J.Y. Establishment and Analysis of SARS Real-time Quantitative System. Taiwan Epidemiology Bulletin 2003 ; July 25 , Vol. 19 , No. 7 :153-168
  6. Cheng WY, Wang S.F, Yang C.Y, Chen H.Y. Genotype Analysis of Measles Virus ,2002. Taiwan Epidemiology Bulletin 2003 ; Oct 25 , Vol. 19 , No. 10 : 231-243.
  7. Wang S.F , Yang J.Y ,Chen H.Y. Analysis and testing for AIDS in 92 Homosexuals at a Private Party. Taiwan Epidemiology Bulletin 2004.,May 25, Vol.20, No.5: 105-120
  8. Wang S.F, Liu S.C, Lee H.C, Wu P.J, Hsu C.C, Wang S.H, Huang Y.F, Tsai S.F, Yang J.Y, Chen H.Y Molecular Biology Confirmation and Analysis of Suspected Transfusion-Associated HIV Cases. Taiwan Epidemiology Bulletin 2004.,Dec. 25, Vol.20, No.12: 281-303.
  9. Wang S.F, Lu S.J, Lee H.C, Wang S.Z, Shu C.J, Wang S.H, Huang Y.F, Tsai S.F, Yang J.Y, Chen H.Y Subtype Identification and Drug-Resistant Point Mutation of Human Immunodeficiency Virus Type I in Taiwan. Taiwan Epidemiology Bulletin 2005.,Apr. 25, Vol.21, No.4: 91-115.
  10. Wang S.F, Lin T.L, Hsu C.C, Lee H.C, Luo S.J, Huang C.W, Yang J.Y, Chen H.Y An Epidemiological Analysis of Enterovirus 71: Taiwan, 1998-2004. Taiwan Epidemiology Bulletin 2005.,May. 25, Vol.21, No.5: 125-153.

專書著作

  1. Sheng-Fan Wang and Yi-Ming Arthur Chen. The Interactions between Avian H5N1 Influenza Virus and Dendritic Cell-Specific ICAM-3 Grabbing Non-Integrin (DC-SIGN). Chapter 6, Global View at Fight against Influenza, Nova Science Publishers.
  2. Sheng-Fan Wang and Yi-Ming Arthur Chen. The Emergence of HIV-1 Circulating Recombinant Form 07_BC Strains in Taiwan. Emerging Infectious Diseases: Global Trends, Surveillance and Eradication, Nova Science Publishers.
  3. 醫學分子檢驗 ( Molecular Diagnosis Medicine), 王聖帆等 著作 五南出版社

研討會發表論文

  1. S.F Wang , W.W.Wong , Y.J.Chan and W. T. Liu. Genotyping and Phenotyping of Human Immunodeficiency Virus Type 1 by Protease Inhibitor Testing. XIV International AIDS Conference 2002 , Barcelona.
  2. S.F Wang, Yang J.Y and Liu W.T. Propagation of SARS Coronavirus with human promonocyte cell line, HL-CZ. The 6 th Asia Pacific Congress of Medical Virology , Nov 2003.
  3. S.F Wang, J.C. Huang, P. Chong, Y.M Chen. DC-SIGN Mediates Avian H5N1 Influenza Virus Infection in Cis and in Trans. Taiwan Society and Microbilogy and 11th Western Pacific Congress on Chemotherpay and Infectious Disease (WPCCID) (Nov.29-Dec.9, 2008)
  4. Y.M Lee, S.F Wang, Y.J Chan, W.T Liu, YM. Chen. Virological investigation of four outbreaks of influenza B reassortants in the northern region of Taiwan from October 2006 to February 2007. Taiwan Society and Microbilogy and 11th Western Pacific Congress on Chemotherpay and Infectious Disease (WPCCID) (Nov.29-Dec.9, 2008).
  5. S.F Wang, Y.M Lee, J.C Huang, Y.M Chen. Phylogenetic and antigenic analysis of human influenza A virus hemagglutinin gene during 1980-2006 in Taiwan. The 8th Asia-Pacific Congress of Medical Virology (Hong Kong, 25-28 Feb 2009).
  6. S.F Wang, L.Yao, S.J Liu, P. Chong, Y.M.Chen, J. Huang, W.T Liu. Identifying the B cell and DR-1501 restricted CD4+ T cell epitopes of Hemagglutinin proteins in the avian H5N1 virus. BIT’s 1st World Congress of Virus and Infections 2010 (July 31-August 3, 2010 Venue: Busan Exhibition & Convention Center, Korea)
  7. S.-F. Wang, C.-H. Tsao, Y.-T. Lin, M.-L. Chiang, F.-C. Chien, P. Chen, D. Hsu, Y.-M. Chen, H.-Y. Chen, F.-T. Liu. Galectin-3 promotes virus budding and cell-to-cell transmission of HIV-1. 7thIAS Conference on Pathogenesis, Treatment and Prevention (Jul 30-Jun 3, 2013, Kuala Lumpur Malaysia)
  8. Sheng-Fan Wang, Ching-Han Tsao, Meng-Lin Chiang, Daniel K. Hsu, Yi-Ming Arthur Chen, Huan-Yuan Chen, Fu-Tong Liu. Galectin-3 promotes HIV-1 cell-to-cell transmission via up-regulating GM1 ganglioside in lipid raft of CD4+ T cells. 8th IAS Conference on Pathogenesis, Treatment and Prevention (19-22 July, 2015, Vancouver, Canada).
  9. Sheng-Fan Wang,* Wen-Hung Wang, Ko Chang, Yen-Hsu Chen, Sung-Pin Tseng, Chia-Hung Yen, Deng-Chyang Wu, and Yi-Ming Arthur Chen. Severe dengue type one outbreak in Taiwan in 2014. 2016 Congress on HIV and Emerging Infectious Diseases(ISHEID) ( 25-27 July, Marseille, France).
  10. Sheng-Fan Wang*, Szu-Wei Huang, Wen-Hung Wang , Chia-Hung Yen, Yi-Wei Hong, Yi-Ming Arthur Chen. HIV-1 CRF07_BC displays slow disease progression and delayed type of viral budding. 2016 International AIDS Conference (July 18-22, Durban, South Africa, 2016).

指導學生獲獎紀錄

時間

參與之國內外研討會

指導之學生

20141214

21屆微生物年會暨學術研討會

碩士生:

洪浥瑋

2015125-28

U.S. Japan Cooperative Medical Sciences Program 17th International Conference on EID

碩士生:

洪浥瑋

2015612

APCMV international Congress

碩士生:

洪浥瑋、

葉峻昇

 

20151125

醫檢學會暨研討會-學生論壇比賽

大學部:

李翠嬋

2016 525-27

International Symposium HIV& Emerging Infectious Disease

碩士生:

洪浥瑋、

葉峻昇

20161211

22屆微生物年會暨學術研討會

碩士生:

  洪浥瑋、

葉峻昇

20161212-14

Frontiers in Glycoscience: Host-pathogen interaction

碩士生:

洪浥瑋、

葉峻昇

 

                       

 

          

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